first_page settings Order Article Reprints Open AccessArticle Multipurpose Impacts of Silver Nitrate on Direct Organogenesis of Begonia rex cv. DS-EYWA via Transverse Thin Cell Layering (tTCL) Technique by Mahboubeh Davoudipahnekolayi 1,* [ORCID] , Delaram Nezamdoost Darestani 2 and Homa Mirshahi 3 1 Department of Phytochemistry and Biochemistry of Natural Compounds, Institute of Chemical, Environmental and Bioscience Engineering, Technische Universität Wien (TU Wien), 1040 Vienna, Austria 2 Department of Breeding and Biotechnology of Horticultural Crops, Zanjan University, Zanjan 45195-313, Iran 3 Department of Horticultural Science, Ferdowsi University of Mashhad, Mashhad 91775-1364, Iran * Author to whom correspondence should be addressed. Horticulturae 2024, 10(9), 986; https://doi.org/10.3390/horticulturae10090986 Submission received: 26 July 2024 / Revised: 8 September 2024 / Accepted: 9 September 2024 / Published: 18 September 2024 (This article belongs to the Special Issue The Role of Plant Growth Regulators in Ornamental Plants) Download keyboard_arrow_down Browse Figures Versions Notes Abstract Begonia rex cv. DS-EYWA is an important plant for indoor and outdoor cultivation, and cv. DS-EYWA is a rare unique cultivar with curly, colorful leaves. Due to their importance, applying plant tissue culture techniques for mass and healthy production in a short period of time without seasonal limitation is of immense economic value. Applying several concentrations of silver nitrate (AgNO3) in combination with varied concentrations of cytokinins including 6-benzylaminopurine (BAP), thidiazuron (TDZ) (0, 0.5, 1, 1.5 mgL−1), and 1-naphthaleneacetic acid (NAA) auxin (0, 0.5, 1 mgL−1) via focusing on transverse thin cell layer (tTCL) petiole explants for high-scale production was used to establish an efficient in vitro propagation protocol. Our results showed that even low concentrations (25 mgL−1) can control internal bacterial infection and increase shoot direct regeneration efficiency. A combination of 1.5 mgL−1 BAP, 0.5 mgL−1 NAA, and 25 mgL−1 AgNO3 was the best treatment to increase the number of direct regenerated shoots, and a lower concentration of BAP (0.5 mgL−1) can be suggested for shoot elongation. Elongated shoots were successfully rooted in MS basal medium and acclimatized in a 1:1 peat moss/perlite sterilized pot mixture

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