The biological species concept defines species as groups of actually or potentially interbreeding natural populations that are reproductively isolated from other such groups (Mayr, 1942). Reproductive isolation, whether prezygotic or postzygotic, plays a central role in maintaining species boundaries. However, hybridization between closely related taxa can challenge these boundaries and provide insight into speciation, gene flow, and evolutionary processes (Coyne and Orr, 2004). High-throughput sequencing enables precise identification of hybrids and determination of parental origins, improving accuracy in studying natural hybridization (Ottenburghs et al., 2017; Toews et al., 2022).

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