Title : ( Optimization and characterization of microbial transglutaminase production from Streptomyces fradiae and its usage in the dairy industry and medical applications )
Authors: Fateme Merajian , Ahmad Asoodeh ,
Abstract
In this study, a non genetically modified strain of Streptomyces fradiae was cultured on ISP medium No. 4 medium supplemented with 1 % glycerol, casein, peptone, and yeast extract, and incubated at pH 7.2 and 27 °C for 7 days. Microbial transglutaminase (MTG) was purified using Q-Sepharose chromatography, and its molecular weight was determined to be 35.6 kDa via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified MTG exhibited optimal activity at pH 8.5 and 50 °C. The enzyme\\\'s half-life was 88.12 ± 0.32 min at 30 °C and 49 ± 0.8 min at 50 °C. Additionally, the Kₘ and Vₘₐₓ values of the MTG reaction were 0.74 μM and 100.5 U/mg, respectively. The addition of 1 % MTG to the yogurt production process significantly reduced the pH from 4.8 to 4.5. Furthermore, syneresis decreased from 2.8 % to 1.5 %, indicating an improvement in the structure and quality of the treated yogurt. The isolated MTG significantly reduced blood clotting time in vitro from 457 ± 2 to 265 ± 2.5 s. In vivo, enzyme application led to a significant reduction in hemostasis time and blood loss in liver tissue. Moreover, histological observations revealed reduced liver damage and hemorrhage after 7 days. Overall, this study first reports that MTG from S. fradiae exhibits great potential as a biocatalyst in both the dairy industry and medical applications.
Keywords
Microbial transglutaminase; Streptomyces fradiae; enzyme characterization; syneresis; blood clotting; histology@article{paperid:1104594,
author = {Merajian, Fateme and Asoodeh, Ahmad},
title = {Optimization and characterization of microbial transglutaminase production from Streptomyces fradiae and its usage in the dairy industry and medical applications},
journal = {International Journal of Biological Macromolecules},
year = {2025},
month = {October},
issn = {0141-8130},
keywords = {Microbial transglutaminase; Streptomyces fradiae; enzyme characterization; syneresis; blood clotting; histology},
}
%0 Journal Article
%T Optimization and characterization of microbial transglutaminase production from Streptomyces fradiae and its usage in the dairy industry and medical applications
%A Merajian, Fateme
%A Asoodeh, Ahmad
%J International Journal of Biological Macromolecules
%@ 0141-8130
%D 2025