Identification of genes affecting production is one of the important priorities in animal breeding strategies. The introgression of genes with a larger effect on quantitative traits into livestock can improve protein and economic resources. The present study aimed to assess high resolution melting (HRM) analysis to genotype bone morphogenetic protein receptor type 1B (Fecundity Booroola gene or FecB) in sheep and myostatin (MSTN) in cattle. This study was conducted on blood samples from 19 cattle and 170 sheep including: 19 control sample of approved FecB genotypes and 151 sample with unknown genotypes. After DNA extraction from blood and semen samples, a 176 bp fragment from exon III of MSTN gene from cattle samples and a 140 bp fragment from exon VIII of FecB gene from sheep samples were amplified. HRM was optimized and conducted in all samples then, the PCR-RFLP and sequencing methods were done to prove the HRM genotyping results. The results obtained by the HRM method was completely identical to those obtained by the sequencing and PCR-RFLP methods, which confirmed the effectiveness of genotyping by HRM method and the possibility of quick and precise genotyping of individuals with FecB and MSTN mutations. Melting temperature range for MSTN genotypes was between 80.90 and 81.60 ºC and for FecB genotypes was between 79.6 and 80.50 ºC. Utilizing the HRM method for genotyping will provide researchers and commercial animal producer’s access to a low cost, and easier method to genotype for any SNP detection. In this study, we have successfully setup a genotyping method for two loci in cattle and sheep economically important genes by high resolution melting method. This method could be used in a large sample size with a high level of confidence.

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