Title : ( Immunomodulatory effects of primed amniotic fluid-derived mesenchymal stem/stromal cells with IFN-γ from unexplained recurrent miscarriage sources )
Authors: Seyed Mehdi Hoseini , Ahmad Reza Bahrami , Seyed Mehdi Kalantar , Mohammad Hasan Sheikhha , Behrouz Aflatoonian , Nasrin Ghasemi , Elham Sadat Hosseini , Maryam Moghaddam Matin , Fateme Montazeri ,
Full TextAbstract
The immunomodulatory properties of mesenchymal stem/stromal cells (MSCs) are strongly influenced by IFN-γ priming and may vary depending on their tissue of origin. This study investigates whether the immunoregulatory responses of human amniotic fluid-derived MSCs (hAF-MSCs) to IFN-γ are altered when derived from individuals with unexplained recurrent pregnancy loss (RPL). We analyzed 10 well characterized hAF-MSC clones, equally divided into non-RPL and RPL groups, to assess IFN-γ-induced expression of immunomodulatory genes. Additionally, we evaluated the paracrine effects of MSCconditioned medium on T regulatory (Treg) cell induction and macrophage polarization. Significant differences were observed between the two groups in the expression of immunomodulatory markers following IFN-γ priming, as well as in their capacity to induce Treg cells and modulate M1/M2 macrophage polarization. These findings support the hypothesis that dysregulated immune pathways contribute to unexplained RPL by revealing impaired IFN-γ responsiveness in hAF-MSCs from fetuses derived from couples with the history of RPL.
Keywords
, Amniotic fluid, Mesenchymal stem/stromal cells, Paracrine factors, Immunomodulatory capacity, Recurrent pregnancy loss
@article{paperid:1106646,
author = {Hoseini, Seyed Mehdi and Bahrami, Ahmad Reza and سیدمهدی کلانتر and محمدحسن شیخها and بهروز افلاطونیان and نسرین قاسمی and الهام سادات حسینی and Moghaddam Matin, Maryam and فاطمه منتظری},
title = {Immunomodulatory effects of primed amniotic fluid-derived mesenchymal stem/stromal cells with IFN-γ from unexplained recurrent miscarriage sources},
journal = {Scientific Reports},
year = {2026},
volume = {16},
number = {1},
month = {January},
issn = {2045-2322},
keywords = {Amniotic fluid; Mesenchymal stem/stromal cells; Paracrine factors; Immunomodulatory capacity;
Recurrent pregnancy loss},
}
%0 Journal Article
%T Immunomodulatory effects of primed amniotic fluid-derived mesenchymal stem/stromal cells with IFN-γ from unexplained recurrent miscarriage sources
%A Hoseini, Seyed Mehdi
%A Bahrami, Ahmad Reza
%A سیدمهدی کلانتر
%A محمدحسن شیخها
%A بهروز افلاطونیان
%A نسرین قاسمی
%A الهام سادات حسینی
%A Moghaddam Matin, Maryam
%A فاطمه منتظری
%J Scientific Reports
%@ 2045-2322
%D 2026
