Objectives: 1- Is there any leptospiral sero infection in dairy cattle herds and their employees? 2- Which leptospira serovar, are the causative agents for dairy cattle herds and their employees? Design: Cross sectional prospective study. Cases: Dairy cattle herds employees and cows. Methods: A total of 551 serum samples 162 workers and 389, owe were collected from 18 dairy herds. Serum samples were tested for Leplospira Interrogans antibody using Microscopic Agglutination test (MAT) by using 22 alive leptospira antigens. Statistical analysis: Chi2 test. Results: Ninety-three (23.9%) cows’ sera and 23(14.19%) employees’ sera showed positive serological reaction against antigens. Thirty-two (34.4%) sera bovine and 7(30.4%) human sera reacted to more than one serovar. The minimum MAT titer was 1:200 and the highest were 1:6400 & 1:800 for cows and employees, respectively. The most common serovar was grippotyphosa grippolyphosa. Conclusion: It is likely that cows and farms employees to be infected with more than one serovar. leptospirosis is becoming increasingly recognized as an occupational disease of farm workers. On the other hand. It was higher Among milkier due to humidity and shedding it with urine and milk during milking times.

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