Proteomics Clinical Applications, ( ISI ), Year (2005-5)

Title : Effects of salinity levels on proteome of suaeda aegyptiaca leaves ( Effects of salinity levels on proteome of suaeda aegyptiaca leaves )

Authors: Mohammad Kafi ,

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Saline soils are the major problem of cultivated lands of Iran. Suaeda aegyptiaca is a salt-tolerant plant (halophytes) that grow naturally in salt-affected areas of Iran. We have employed proteomics to identify the mechanisms of salt responsiveness in leaves of S. aegyptiaca grown under different salt concentrations. Ten-day-old plants were treated with 0, 150, 300, 450, and 600 mM NaCl. After 30 days of treatment, leaf samples were collected and analyzed using 2-D-PAGE. Out of 700 protein spots reproducible detected within replications, 102 spots showed significant response to salt treatment compared to 0 mM NaCl. We analyzed expression pattern of saltresponsive proteins using a hierarchical and two nonhierarchical (Fuzzy ART and SOM) statistical methods and concluded that Fuzzy ART is the superior method. Forty proteins of 12 different expression groups were analyzed using LC/MS/MS. Of these, 27 protein spots were identified including proteins involved in oxidative stress tolerance, glycinebetain synthesis, cytoskeleton remodeling, photosynthesis, ATP production, protein degradation, cyanide detoxification, and chaperone activities. The expression pattern of these proteins and their possible roles in the adaptation of S. aegyptiaca to salinity is discussed.

Keywords

Halophyte / Oxidative stress / Salinity / Suaeda aegyptiaca / Two dimensional
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@article{paperid:203715,
author = {Kafi, Mohammad},
title = {Effects of salinity levels on proteome of suaeda aegyptiaca leaves},
journal = {Proteomics Clinical Applications},
year = {2005},
month = {May},
issn = {1615-9853},
keywords = {Halophyte / Oxidative stress / Salinity / Suaeda aegyptiaca / Two dimensional electrophoresis},
}

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%0 Journal Article
%T Effects of salinity levels on proteome of suaeda aegyptiaca leaves
%A Kafi, Mohammad
%J Proteomics Clinical Applications
%@ 1615-9853
%D 2005

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