Plant and Cell Physiology, ( ISI ), Volume (48), No (3), Year (2007-3) , Pages (441-450)

Title : Phosphoenolpyruvate Carboxykinase in Arabidopsis: Changes in Gene Expression, Protein and Activity d ( Phosphoenolpyruvate carboxykinase in Arabidopsis: changes in gene expression, protein and activity during vegetative and reproductive development. )

Authors: S. Malone , ZH. Chen , Ahmad Reza Bahrami , PR. Walker , JE. Gray , RC. Leegood ,

Access to full-text not allowed by authors

Citation: BibTeX | EndNote

The aim of this work was to investigate the occurrence of phosphoenolpyruvate carboxykinase (PEPCK) in different tissues of Arabidopsis thaliana throughout its vegetative and reproductive growth. The A. thaliana genome contains two PEPCK genes (PCK1 and PCK2), and these are predicted to generate 73,404 and 72,891 Da protein products, respectively. Both genes were transcribed in a range of tissues; however, PCK1 mRNA appeared to be more abundant and was present in a wider range of tissues. PEPCK protein was present in flowers, fruit, developing seed, germinating seed, leaves, stems and roots. Two PEPCK polypeptides, of approximately 74 and approximately 73 kDa were detected by immunoblotting, and these may arise from PCK1 and PCK2, respectively. PEPCK was abundant in cotyledons during post-germinative growth, and this is consistent with its well established role in gluconeogenesis. PEPCK was also abundant in sink tissues, such as young leaves, in developing flowers, fruit and seed. Immunohistochemistry and in situ hybridization showed that PEPCK was present in the nectaries, stigma, endocarp of the fruit wall and in tissues involved in the transfer of assimilates to the developing ovules and seeds, such as the vasculature and seed coat. The potential functions of PEPCK in A. thaliana are discussed.

Keywords

, Phosphoenolpyruvate carboxykinase in Arabidopsis: changes in gene expression, protein and activity during vegetative and reproductive
برای دانلود از شناسه و رمز عبور پرتال پویا استفاده کنید.

@article{paperid:204075,
author = {S. Malone and ZH. Chen and Bahrami, Ahmad Reza and PR. Walker and JE. Gray and RC. Leegood},
title = {Phosphoenolpyruvate Carboxykinase in Arabidopsis: Changes in Gene Expression, Protein and Activity d},
journal = {Plant and Cell Physiology},
year = {2007},
volume = {48},
number = {3},
month = {March},
issn = {0032-0781},
pages = {441--450},
numpages = {9},
keywords = {Phosphoenolpyruvate carboxykinase in Arabidopsis: changes in gene expression; protein and activity during vegetative and reproductive development.},
}

[Download]

%0 Journal Article
%T Phosphoenolpyruvate Carboxykinase in Arabidopsis: Changes in Gene Expression, Protein and Activity d
%A S. Malone
%A ZH. Chen
%A Bahrami, Ahmad Reza
%A PR. Walker
%A JE. Gray
%A RC. Leegood
%J Plant and Cell Physiology
%@ 0032-0781
%D 2007

[Download]