Abstract: This study was conducted in three phases for identification of protective antigens in Hyalomma anatolicum anatolicum that can induce the best protection in the sheep and cattle. In the First phase of study, Hyalomma .a.anatolicum was cultured for preparing enough antigens .Then, Four antigenic preparation , Viz, gut supernatant antigen (GSA),gut pellet antigen (GPA) ,Whole tick supernatant antigen (WTSA) and Whole tick pellet antigen (WTPA) were made partially fed adult female Hyalomma.a. anatolicum. Four groups of five rabbits were immunized with the antigens in freund’s adjuvant emulsion and a fifth group was kept as control. The rabbits were challenge with ten pairs of homologus ticks and characteristic representing tick feeding and fertility were recorded and compared between groups. A significant decrease in engorgment weight, Index feeding , weight of egg and Index fertility were observed in GPA immunized rabbits.

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