Title : ( Molecular detectionof potato spindle tuber viroid in Razavi and Northern Khorasan provinces )
Authors: Behrooz Jafarpour ,
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Abstract
The aim of this study was to identify the potato spindle tuber viroid( PSTVd) by reverse transcriptase-PCR . among all plant diseases which are caused viroids, PSTVd was the first viriod which is recognized by plant pathologist . suspected potato tubers from Razavi and Northern Khorasan provinces collected and cultured . leaves of cultured tubers were used for RNA extraction by PEG6000 precipitation method. subsequent RT-PCR reaction has been carried out using specific primers. A 359 bp fragment has been appeared after electrophoresis which has not appeared in healthy tuber samples.
Keywords
, potato, PSTVd, reverse trascriptase-PCR
@article{paperid:1006460,
author = {Jafarpour, Behrooz},
title = {Molecular detectionof potato spindle tuber viroid in Razavi and Northern Khorasan provinces},
journal = {Pakistan Journal of Biological Sciences},
year = {2008},
volume = {11},
number = {12},
month = {August},
issn = {1028-8880},
pages = {1642--1645},
numpages = {3},
keywords = {potato;PSTVd;reverse trascriptase-PCR},
}
%0 Journal Article
%T Molecular detectionof potato spindle tuber viroid in Razavi and Northern Khorasan provinces
%A Jafarpour, Behrooz
%J Pakistan Journal of Biological Sciences
%@ 1028-8880
%D 2008
