G.H. Hashemitabar, G.H. Razmi, A. Sharoozian. Ferdowsi University of Mashhad, Mashhad, Iran Cystic hydatid disease (Hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus. As its diagnosis by clinical symptoms alone is difficult and confusing, but serological diagnostic techniques are used to absolutely confirm the disease. These techniques can also be used for epidemiological studies. The present study was performed with a commercial human ELISA kit for diagnosis of hydatidosis in sera collected from sheep with hydatidosis. Sera were collected from 68 cases of hydatidosis proven by inspection of hydatid infected livers and lungs of the sheep slaughtered in Mashhad abattoir and also from 11 healthy cases. Sera samples were examined by ELISA kit. The results showed that from 68 cases of hydatidosis in sheep, 67 samples had positive adsorbance. Also from 11 healthy samples, 9 of them had negative absorbance value. The sensitivity and specificity of the test were %98/5 and %81/8 respectively. By getting these results, it can be concluded that it is possible to use human ELISA kit for diagnosis of hydatidosis in sheep.

G.H. Hashemitabar, G.H. Razmi, A. Sharoozian. Ferdowsi University of Mashhad, Mashhad, Iran Cystic hydatid disease (Hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus. As its diagnosis by clinical symptoms alone is difficult and confusing, but serological diagnostic techniques are used to absolutely confirm the disease. These techniques can also be used for epidemiological studies. The present study was performed with a commercial human ELISA kit for diagnosis of hydatidosis in sera collected from sheep with hydatidosis. Sera were collected from 68 cases of hydatidosis proven by inspection of hydatid infected livers and lungs of the sheep slaughtered in Mashhad abattoir and also from 11 healthy cases. Sera samples were examined by ELISA kit. The results showed that from 68 cases of hydatidosis in sheep, 67 samples had positive adsorbance. Also from 11 healthy samples, 9 of them had negative absorbance value. The sensitivity and specificity of the test were %98/5 and %81/8 respectively. By getting these results, it can be concluded that it is possible to use human ELISA kit for diagnosis of hydatidosis in sheep.

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