Archives of Razi Institute, ( ISI ), Year (2007-11)

Title : ( Detection of salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran )

Authors: Abdollah Jamshidi , تقی زهرائی صالحی , Samira Afshari Nik ,

Citation: BibTeX | EndNote

Abstract

Summary: A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad . The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping . PCR amplification of invA gene as a specific method for detection of Salmonella was evaluated. In this study Salmonella was isolated from 11.66% of samples by conventional culture method, then in serological test 28.6% of them detected as serogroup B and 71.4% as serogroup C. In this investigation all positive results by conventional culture method were confirmed by PCR amplification. because of rapidity and high specificity and sesitivity of PCR method, by standardization of this method, it could be concidered as an alternative to conventional culture method for cofirmation of Salmonella presence in raw poultry meat.

Keywords

, Poultry meat, Salmonella, PCR, invA gene, conventional culture method
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@article{paperid:1007002,
author = {Jamshidi, Abdollah and تقی زهرائی صالحی and Afshari Nik, Samira},
title = {Detection of salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran},
journal = {Archives of Razi Institute},
year = {2007},
month = {November},
issn = {0365-3439},
keywords = {Poultry meat; Salmonella; PCR; invA gene; conventional culture method},
}

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%0 Journal Article
%T Detection of salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran
%A Jamshidi, Abdollah
%A تقی زهرائی صالحی
%A Afshari Nik, Samira
%J Archives of Razi Institute
%@ 0365-3439
%D 2007

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