In this study we report that each individual adult (finally differentiated) tissue has a signature chromatin organization. However, cells in culture do not demonstrate a remarkable chromatin configuration. By relating to the number of active genes acquired from high-throughput transcriptome analyses, we demonstrate that the final organization of genome in the nuclear chromatin is not determined by the number of active and silenced genes. We also provide evidence for the lattice model of chromatin organization (Dehghani et al., 2005) by demonstrating that chromosome territories are not demarcated by \\\\\\\"interchromatin channels\\\\\\\" and in fact they are intermingled. Also, we show that transcription of genes is localized to the chromatin fibers regardless of their location within or at the periphery of chromosome territories. These data provide evidence for the potential of spatial organisation of chromatin and sub-nuclear organisation in the regulation of gene activity and cellular differentiation.

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