The aim of present study was to prepare human serum albumin (HSA) nanoparticle for drug delivery. These nanoparticles were prepared by desolvation method. The body distribution of colloidal drug delivery systems was mainly influenced by two physicochemical properties namely particle size and surface characteristics. The influence of several factors on the fabrication process including the pH value, HSA concentration [mg/ml], glutaraldehyde concentration [%], agitation speed [rpm] and the ratio of organic solvent/HSA solution were investigated herein. The smallest size of nanoparticles was 69 nm and the largest size was 424 nm which is suitable for drug delivery. The crosslinker concentration [%] and agitation speed [rpm] showed that have less effect on produced nanoparticle size. For characterizing the nanoparticle sample atomic force microscopy (AFM) and scanning electron microscopy (SEM) was employed whilst size distribution was measured by photon correlation spectroscopy (PCS). It was shown that the produced nanoparticles were almost formed by a smooth surface. Based on these characteristics, HSA nanoparticles were good enough to be candidate for drug delivery. The mechanistic of the optimum conditions for preparing protein nanoparticle as well as their characterization are discussed.

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