Pakistan Journal of Biological Sciences, ( ISI ), Volume (11), No (23), Year (2008-12) , Pages (2629-2633)

Title : ( Evaluation of Genetic Diversity among Different Genotypes of Brassica napus using Rando Amplified Polymorphic DNA Marker )

Authors: Esmaeil Fazelisangari , Farajollah Shahriari Ahmadi , حسین سمیع زاده , Abdolreza Bagheri , Mohammad Farsi ,

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Abstract: In current research, genetic relationships among rapeseed genotypes from several geographical origins including France, Canada, Germany, Iran, Hungary, Denmark, Australia and America were evaluated using RAPD markers. Among generated 86 bands, 80 different polymorphic bands were obtained using 9 random primers. Diversity Index (DI) or Polymorphism Information Content(PIC) was varied from 0.29 to 0.48, showed a relatively high potential of primers among studied genotypes. Dice similarity coefficients between genotypes was calculated using Nei and Li formula. Maximum(0.91) and minimum(0.42) similarity coefficients were observed between Bristol and Amber genotypes, consul snd expres, repectively. Cluster analysis based on dice similarity coefficient was also carried out. Based on the cluster analysis, genotypes were grouped into five main clusters, Results showed that genotypes with same geographical origin were genetically different. Therefore, geographical origins of genotypes cannot be used as a base to cross parent to obtain high heterosis and it must be carried out by exact genetic studies. Results confirmed that RAPD is a simple, cheap and fast method for evaluation of genetic diversity of Brassica napus.

Keywords

, Key word: Brassica napus, cluster analysis, genetic diversity, RAPD
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@article{paperid:1010714,
author = {Fazelisangari, Esmaeil and Shahriari Ahmadi, Farajollah and حسین سمیع زاده and Bagheri, Abdolreza and Farsi, Mohammad},
title = {Evaluation of Genetic Diversity among Different Genotypes of Brassica napus using Rando Amplified Polymorphic DNA Marker},
journal = {Pakistan Journal of Biological Sciences},
year = {2008},
volume = {11},
number = {23},
month = {December},
issn = {1028-8880},
pages = {2629--2633},
numpages = {4},
keywords = {Key word: Brassica napus; cluster analysis; genetic diversity; RAPD marker},
}

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%0 Journal Article
%T Evaluation of Genetic Diversity among Different Genotypes of Brassica napus using Rando Amplified Polymorphic DNA Marker
%A Fazelisangari, Esmaeil
%A Shahriari Ahmadi, Farajollah
%A حسین سمیع زاده
%A Bagheri, Abdolreza
%A Farsi, Mohammad
%J Pakistan Journal of Biological Sciences
%@ 1028-8880
%D 2008

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