Title : ( The effect of supplementated diet by sucrose and/or starch on fibrobacter succinogenes population in ruminal fluid of Holstein steers determined by real-time PCR )
Authors: Mohsen Danesh Mesgaran , , Seyed Alireza Vakili , Alireza Heravi Moussavi ,
Full TextAbstract
The objective of the present study was to investigate the effect of diets containing different types of non- polymerase chain reaction (RT-PCR). Four Holstein steers were used in a 4×4 Latin square design (21 days each period). A basal diet (BD) was formulated to be contained of alfalfa hay, barley grain, soybean meal of sucrose and starch (SuSt) was added to the BD at the rate of 70 g/kg DM. Diets were offered as 2-2.5 the morning feeding at the last day of each period. Samples were analyzed for FBS quantitation by qPCR. DNA was extracted from the samples using the QIAamp® DNA stool mini kit (Qiagen Ltd, Crawley, West Sussex, UK). Fibrobacter succinogenes rDNA concentration was measured by RT-PCR relative to conditions were 95
Keywords
, sucrose, starch, fibrobacter succinogenes, iruminal fluid, Holstein steers, real-time PCR
@inproceedings{paperid:1011960,
author = {Danesh Mesgaran, Mohsen and , and Vakili, Seyed Alireza and Heravi Moussavi, Alireza},
title = {The effect of supplementated diet by sucrose and/or starch on fibrobacter succinogenes population in ruminal fluid of Holstein steers determined by real-time PCR},
booktitle = {60th Annual Meeting of the European Association for Animal Production},
year = {2009},
keywords = {sucrose; starch; fibrobacter succinogenes;iruminal fluid; Holstein steers; real-time PCR},
}
%0 Conference Proceedings
%T The effect of supplementated diet by sucrose and/or starch on fibrobacter succinogenes population in ruminal fluid of Holstein steers determined by real-time PCR
%A Danesh Mesgaran, Mohsen
%A ,
%A Vakili, Seyed Alireza
%A Heravi Moussavi, Alireza
%J 60th Annual Meeting of the European Association for Animal Production
%D 2009
