Human Serum Albumin (HSA) nanoparticles represent promising drug carrier systems. Particle size is a crucial parameter in particular for the in vivo behavior of nanoparticles after intravenous injection. The object of present study was to characterize the desolvation process of HSA for preparation of nanoparticles. Two process parameters were examined to achieve a suitable size of nanoparticles such as the pH value and the amount of glutaraldehyde concentration (%).The smallest size of nanoparticles achieved was 91 nm and the largest size was 388 nm which is suitable for drug delivery. The pH value of the HSA solution prior to the desolvation procedure was identified as the major factor determining particle size and the amount of crosslinker showed that it has less efect on produced nanoparticle size. The nanoparticle sample was purified by five cycles centrifugation (20 000 g, 8 min) and redispersion of the pellet to the original volume in 10 mM NaCl at pH values of 7.5-9, respectively, and then analyzed by particle size analyzer (PCS).

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