Toxicon, ( ISI ), Volume (39), No (12), Year (2001-12) , Pages (1871-1882)

Title : ( The facilitatory actions of snake venom phospholipase A2 neurotoxins at the neuromuscular junction are not mediated through voltage-gated K+ channels )

Authors: Behrooz Fathi Hafshejani , E. G. Rowan , A. L. Harvey ,

Citation: BibTeX | EndNote

Abstract

Electrophysiological investigations have previously suggested that phospholipase A(2) (PLA(2)) neurotoxins from snake venoms increase the release of acetylcholine (Ach) at the neuromuscular junction by blocking voltage-gated K(+) channels in motor nerve terminals.We have tested some of the most potent presynaptically-acting neurotoxins from snake venoms, namely beta-bungarotoxin (BuTx), taipoxin, notexin, crotoxin, ammodytoxin C and A (Amotx C & A), for effects on several types of cloned voltage-gated K(+) channels (mKv1.1, rKv1.2, mKv1.3, hKv1.5 and mKv3.1) stably expressed in mammalian cell lines. By use of the whole-cell configuration of the patch clamp recording technique and concentrations of toxins greater than those required to affect acetylcholine release, these neurotoxins have been shown not to block any of these voltage-gated K(+) channels. In addition, internal perfusion of the neurotoxins (100 microg/ml) into mouse B82 fibroblast cells that expressed rKv1.2 channels also did not substantially depress K(+) currents. The results of this study suggest that the mechanism by which these neurotoxins increase the release of acetylcholine at the neuromuscular junction is not related to the direct blockage of voltage-activated Kv1.1, Kv1.2, Kv1.3, Kv1.5 and Kv3.1 K(+) channels.

Keywords

, beta-bungarotoxin (BuTx), taipoxin, notexin, crotoxin, ammodytoxin , voltage-gated K(+) channels, release of acetylcholine
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@article{paperid:1015364,
author = {Fathi Hafshejani, Behrooz and E. G. Rowan and A. L. Harvey},
title = {The facilitatory actions of snake venom phospholipase A2 neurotoxins at the neuromuscular junction are not mediated through voltage-gated K+ channels},
journal = {Toxicon},
year = {2001},
volume = {39},
number = {12},
month = {December},
issn = {0041-0101},
pages = {1871--1882},
numpages = {11},
keywords = {beta-bungarotoxin (BuTx); taipoxin; notexin; crotoxin; ammodytoxin ;voltage-gated K(+) channels; release of acetylcholine},
}

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%0 Journal Article
%T The facilitatory actions of snake venom phospholipase A2 neurotoxins at the neuromuscular junction are not mediated through voltage-gated K+ channels
%A Fathi Hafshejani, Behrooz
%A E. G. Rowan
%A A. L. Harvey
%J Toxicon
%@ 0041-0101
%D 2001

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