Blood samples were collected from 60 great sturgeons, Huso huso, to establish the following serum enzyme activity: aspartate amino-transferase (AST), alanine amino-transferase (ALT), lactic acid dehydrogenase (LDH), creatine kinase (CK), and alkaline phosphatase (ALP) using an autoanalyzer, and acid phosphatase (ACP) by manual method. Thirty 5-year-old cultured fish were caught from each of two sites; a brackish-water earth pond in Bafgh and a freshwater pond in Gorgan in the centre and northeast of Iran, during May 2006. Results of the serum enzymes activity for H. huso samples from Bafgh and Gorgan were: AST, 502.9±258.2 and 436.1±186.8; ALT, 104.4±35.1 and 53.1±38.7; LDH, 3094.2±1277.5 and 2486.3±1393.3; CK, 3632.9±2618.7 and 3967±5054.9; ALP, 281.2±112.7 and 762.2±600.2; ACP, 13.3±2.5 and 33±6.8 IU/L. Mean values of ALT, ALP and ACP were significantly different in the fish from the two sites (p<0.05). These results may be used to understand some biological (e.g., serum enzyme activity) and ecological characteristics of cultured H. huso. Keywords Sturgeon . Huso huso . Culture . Serum enzymes . Iran Introduction

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