Helicobacter pullorum was originally isolated from the feces and damaged livers of broilers and laying hens. It was defined as a new species in 1994. This organism has also been isolated from humans with gastroenteritis. In this study a total of 100 intestinal content of broilers carcasses from 10 flocks at a poultry abattoir in Mashhad suburb were tested for the presence of Helicobacter pullorum by conventional culture method using a combination of supplemented brain heart infusion agar and a modified filter technique and identification were performed using a polymerase chain reaction amplifying a 447-bp fragment of the 16S rRNA gene of H. pullorum. Four isolates from intestinal samples from 4 different flocks were isolated. The results show the presence of H. pullorum live chickens in this aria of Iran and may represent a risk to human health.

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