Anthurium andreanum Var. tera from family Aracease is a very beautiful and one of the most expensive flowers in the world, which under normal condition reproduce annually. Spathe of this flower has heart-shaped appearance and red, pink, green colors. Proliferation with seeds which used for increment previously abolished for some reason, such as less speed of growth and susceptibility of plant to microbial and virulent agents. The study presented in this paper was intended to establish a reproducible protocol for indirect In vitro regeneration of this plant. For this purpose, we investigated the effects of different 2,4-D and BAP hormone treatments- together or alone- on callus induction and regeneration of leaf explants. Among different media, 0.1 mg/lit 2,4-D + 1.5 mg/lit BAP and 0.15 mg/lit 2,4-D + 1.5 mg/lit BAP produced callus with the highest fresh and dry weight. The most esponsive shooting and rooting was achieved with transferring produced calli on MS medium without any growth regulators. The whole plantlets then transferred to greenhouse for further development.

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