An experiment was conducted to study the effects of supplementary extruded cotton seed (ECOS) or canola seed (ECAS) on the composition of unsaturated fatty acids in plasma, erythrocytes and liver of Mehraban male lambs. The treatments included: (1) control (C); (2) diet C+6% ECAS, (3) diet C+6% ECOS, (4) diet C+12% ECAS, (5) diet C+12% ECOS, (6) diet C+6% ECAS+6% ECOS, (7) diet C+12% ECAS +6% ECOS, (8) diet C+6% ECAS +12% ECOS, (9) diet C+12% ECAS+12% ECOS, (10) diet C+18% ECAS+18% ECOS. A completely randomized design experiment was applied to feed the 10 dietary treatments to 60 individually pen housed lambs, to have 6 lambs per diet. The average weight of 5–6 mo lambs at the commence of study was 34.3 ± 2.12 kg. The experiment lasted for 90 d. The amount of oleic acid, linoleic acid and linolenic acid in plasma and oleic acid, and linolenic acid in erythrocytes lipids were higher in lambs fed diet contained either or a combination of supplemental extruded oil seeds compared with those fed control diet. The supplementation of 6% or 12% ECAS significantly increased linoleic acid in liver lipids compared with those fed diet containing ECOS. There was a linear (P < 0. 001) correlation between the levels of dietary ECAS or ECOS with linoleic and linolenic acids content of liver.

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