Plant performance and essential oil content and chemical composition, may vary due to a variety of factors, such as plant genotype, environmental conditions and plant age. The aim of the present study is to evaluate amounts and chemical composition of the essential oil of Achillea millefolium subsp millefolium in two successive years. The seeds of this species were cultivated on September 2006 and the flowering parts of the plants harvested on July 2007 and 2008 at full flowering stage. After drying, essential oils of the samples extracted and analyzed by GC and GC-MS. The results showed that essential oils yield of A. millefolium in the first and second year after sowing were 0.15 % and 0.5% (v/w) respectively. The major compound in the first year samples were 1,8-cineole (14.93%), Elemodiol 8-α-11 (9.74), Sabinene (8.46%) and Oplopanone (5.12%) while in the second year Camphor (25.54%), 1,8-cineole (7.27%), Germacrene B (6.18%) and Globulol (4.94%) included the major components.

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