Title : ( A preliminary molecular study of the Iranian species of Calomyscus (Rodentia- Calomyscidae) using RFLP )
Authors: , Jamshid Darvish , E. RASTEGAR-POUYANI , Roohollah Siahsarvie , Safieh Akbari rad ,
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Abstract
The Calomyscidae is a small family of Rodentia that has recently been classified into 8 species in a single genus, Calomyscus. Currently 5 species are reported in Iran: C. elburzensis,، C. bailwardi، C. grandis ، C. urartensis and C. hotsoni. Species discrimination based on morphological characteristics is difficult, so this study re-evaluated the phylogenetic relationships and taxonomic status of the morphotypes within Calomyscus using PCRRFLP as a molecular marker. Forty-seven samples of Calomyscus spp from 9 geographically separated regions of Iran were analyzed. The cytochrome b gene was amplified by PCR, digested using 3 restriction enzymes, HinfI, HincII and HaeIII, and the patterns separated electrophoretically. Species of long tailed hamster were discriminated from one another. A phylogenetic analysis of the dataset produced well distinguished clades.
Keywords
, Calomyscus, Cytochrome b, RFLP.
@article{paperid:1019232,
author = {, and Darvish, Jamshid and E. RASTEGAR-POUYANI and Siahsarvie, Roohollah and Akbari Rad, Safieh},
title = {A preliminary molecular study of the Iranian species of Calomyscus (Rodentia- Calomyscidae) using RFLP},
journal = {Iranian Journal of Animal Biosystematics},
year = {2009},
volume = {5},
number = {2},
month = {November},
issn = {1735-434X},
pages = {33--41},
numpages = {8},
keywords = {Calomyscus; Cytochrome b; RFLP.},
}
%0 Journal Article
%T A preliminary molecular study of the Iranian species of Calomyscus (Rodentia- Calomyscidae) using RFLP
%A ,
%A Darvish, Jamshid
%A E. RASTEGAR-POUYANI
%A Siahsarvie, Roohollah
%A Akbari Rad, Safieh
%J Iranian Journal of Animal Biosystematics
%@ 1735-434X
%D 2009
