Transforming materials into nano scale will change their physical, chemical, biological characteristics and catalytic activities. Specific surface area of most of materials at nano scale will increase their chemical and biological activities. Thus, new properties will appear in nano particles such as, more solubility, chemical activities and the ability of penetration into cell membrane. Plant iron deficiency is very common and prevalent nutrient deficiency symptom, especially in calcareous soils. The present research was conducted to study the nano iron oxide particles efficiency on Fe, Mn, Zn and Cu concentrations in wheat plant. This experiment was carried out with two types of nano (25-250 nm) and normal (0.02-0.06 mm) iron oxides (pH= 2.4), each with five concentration levels (0, 0.05, 0.1, 0.5 and 1%) in a completely randomized design (factorial), with three replications in greenhouse of Agriculture College, Ferdowsi University of Mashhad. Results showed that the application of both types of iron oxides increased Fe, Zn and Cu concentrations in soil. There was a decreasing trend in plant Mn concentration, while plant Fe, concentrations were increased with increasing the amounts of iron oxides in soil. The increasing plant Fe, Zn and Cu concentrations was more pronounced with the application of nano iron oxide than normal iron oxide in wheat plant. Therefore, nano iron oxide was superior to normal iron oxide, with respect to, Fe concentration in wheat plant, but more research are needed to justify the proper application methods and amount of nano iron oxide.

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