Title : ( Molecular Identification of ovine theileria species in ferdos (south khorasan province) east of Iran. )
Authors: Khazrainia P , Haddadzadeh HR , Kazemi B , Mohammad Heidarpour , Aktas M ,
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Abstract
There are many foci of ovine theileriosis distributed in Iran. Therefore, we investigated the role of different species of theileria in ovine theilerisis in Ferdos, an area endemic for ovine theileriosis in the east part of Iran. For these purepose we used a nested PCR for the amplification of virtually all the species of theileria is described. For differentiation of various theileria spp. We developed a RFLP assay. Blood samples were collected in EDTA from 50 sheep in ferdos east part of Iran and used foe wxtract DNA for PCR analysis. A 1416-bp DNA fragment was specifically amplified from blood samples of naturally infected sheep with theileria spp. For differentiation of various theileria species (T. annulata, T.lestoquardi, T. ovis) RFLP of PCR products of theileria spp. was done. Twenty percent of the samples were positive for theileria spp. using nestred PCR. By RFLP of PCR products, all positive samples were identified as T. lestoquardi. No T. ovis was identified in the samples of this area.
Keywords
, T. lestoquardi, ovine theileriosis, theileria annulata, T. ovis, , Ferdos, Iran.
@inproceedings{paperid:1019826,
author = {Khazrainia P and Haddadzadeh HR and Kazemi B and Heidarpour, Mohammad and Aktas M},
title = {Molecular Identification of ovine theileria species in ferdos (south khorasan province) east of Iran.},
booktitle = {VI interbational conference on ticks and tick borne pathogens},
year = {2008},
keywords = {T. lestoquardi; ovine theileriosis; theileria annulata; T. ovis; ; Ferdos; Iran.},
}
%0 Conference Proceedings
%T Molecular Identification of ovine theileria species in ferdos (south khorasan province) east of Iran.
%A Khazrainia P
%A Haddadzadeh HR
%A Kazemi B
%A Heidarpour, Mohammad
%A Aktas M
%J VI interbational conference on ticks and tick borne pathogens
%D 2008
