The influence of in-ovo administration of aromatase inhibitors on sex differentiation was investigated. Five hundred fertilized breeder eggs of Ross strain were used as animal model in this experiment. Eggs were divided into five groups which were treated with four different antiaromatase inhibitors, namely, tomoxifen (TMX), garlic extract (GAR79), tomato extract (TOM86) and clomiphene citrate (CLS). Moreover, water-injection was considered as control (DW). Eggs were sanitized and prepared for incubation in a regular automatic hatchery. At day 5 of incubation period, eggs injection site were cleaned with 70% ethylic alcohol, bored by a needle and anti- aromatases were injected using insulin syringe through thin end of the eggs and then sealed by melted paraffin. Hatched day old chickens were feather sexed to determine Male/Female ratio. Further study was performed to control 42 day old Chicken’s gonads alteration when treated with different antiaromatases. Results showed that GAR79 with highest significant (p<0.05) anti-aromatase activity affects male production (80.43%), followed by TOM86, TMX and DW respectively. It is concluded that GAR79 and TOM86 are of importance with high potential antiaromatase effects on sex differentiation for different purposes

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