The present study was undertaken to evaluate the effects of autologous Platelet-rich plasma (PRP) on wound healing in dogs. Under general anesthesia, six 2 × 2-cm full-thickness skin wounds were created on the backs of 5 mix-breed male adult dogs symmetrically. In each dog, the 3 wounds on the right side were treated topically with 1.5 ml PRP jelly, whereas left side wounds received no treatment. For macroscopic evaluation, at the days 0, 3, 5, 7, 10, 13, 17, 20, and 24, digital photographs were taken from wounds. At the days 10, 17 and 24 after wounding, biopsy was taken from the center and corner of each wound for hydroxyprolin measurement and histopathologic evaluation respectively. No statistically significant differences were found in percent of wound contraction, epithelialization and healing between test and control group during study (P>0.05). There were no significant differences between median of hydroxyprolin levels, median of inflammatory infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition and collagen bundle formation scores, in the specimens from left and right wounds (P>0.05). The PRP-treated wounds, except in the 3-week study, showed a tendency toward increased epithelization rate in both periods, but no statistical significance was found. The result of the present study demonstrated that topical application of platelet-rich plasma can not accelerate the repair of small granulating wounds on the back of dogs. This treatment may hold more promise for the management of wounds that function or prevention of recurrence is important.

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