@article{paperid:1022719,
author = { -, - and Jamshidi, Abdollah and Bassami, Mohammad Reza},
title = {Isolation and Identification of campylobacter jejuni from poultry carcasses using conventional culture methods and multiplex PCR assay},
journal = {Iranian Journal of Veterinary Medicine},
year = {2011},
volume = {5},
number = {1},
month = {March},
issn = {2251-8894},
pages = {31--35},
numpages = {4},
keywords = {Abstract: Campylobacter jejuni is a major cause of food-borne diarrhea in many countries. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study conventional culture and multiplex PCR methods in detection of Campylobacter jejuni isolated from poultry carcasses was compared. Number of 100 samples; representing 20 broiler flocks; was collected from poultry carcasses after the evisceration stage in the processing line at a commercial broiler slaughtering facility in Mashhad. In conventional culture method; samples were processed by enrichment followed by selective plating; and then suspected colonies were isolated on sheep blood agar and tested for morphology; motility; gram staining; biochemical properties and hippurate hydrolysis activity. For identification of Campylobacter genus and its jejuni serovar by molecular method; a multiplex PCR assay (m-PCR) with two sets of specific primers was used. In hippurate hydrolysis test from suspected colonies; 76% of samples were determined as positive; but in m-PCR assay 28% of cultures harvest; identified as C.jejuni; also two percent of hippurate hydrolyze negative colonies; were positive in m-PCR test. It seems that conventional method; based on hippurate hydrolysis test for detection of C.jejuni; could not be a reliable test. The use of m-PCR method based on amplification from conserved genes; allows reliable detection and identification of C. jejuni.},
}

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