Title : A Ca-independent alpha-amylase that is active and stable at low pH ( A Ca-independent α-amylase that is active and stable at low pH from the Bacillus sp. KR-8104 )
Authors: Reza Hassan Sajedi , Hossein Naderi-Manesh , Khosro Khajeh , Rahim Ahmadvand , Bijan Ranjbar , Ahmad Asoodeh , Fatemeh Moradian ,
Full TextAbstract
Bacillus sp. KR-8104 was selected from a set of 18 bacteria strains isolated from soil samples and screened for production of amylase. The maximum productivity obtained at pH 5–6 and 60–65 h after cultivation in production medium. New extracellular Ca-independent alpha-amylase was highly purified using ion exchange and hydrophobic interaction chromatography, which showed a single band with an apparent molecular weight of 59 kDa by SDS-PAGE. This enzyme is active in a wide pH range with its maximum activity at low pH values (4.0–6.0) and has the 90% of its maximum activity at pH 3.5. The -amylase is optimally active at 75–80 ◦C. The presence or absence of Ca2+ and EDTA did not affect enzyme activity and thermal stability.
Keywords
, Bacillus sp.; Ca, independent , amylase; Alkaline protease; Broad range of pH; pH profile; Industrial applications
@article{paperid:102295,
author = {Reza Hassan Sajedi and Hossein Naderi-Manesh and Khosro Khajeh and Rahim Ahmadvand and Bijan Ranjbar and Asoodeh, Ahmad and Fatemeh Moradian},
title = {A Ca-independent alpha-amylase that is active and stable at low pH},
journal = {Enzyme and Microbial Technology},
year = {2005},
volume = {36},
month = {February},
issn = {0141-0229},
pages = {666--671},
numpages = {5},
keywords = {Bacillus sp.; Ca-independent -amylase; Alkaline protease; Broad range of pH; pH profile; Industrial applications},
}
%0 Journal Article
%T A Ca-independent alpha-amylase that is active and stable at low pH
%A Reza Hassan Sajedi
%A Hossein Naderi-Manesh
%A Khosro Khajeh
%A Rahim Ahmadvand
%A Bijan Ranjbar
%A Asoodeh, Ahmad
%A Fatemeh Moradian
%J Enzyme and Microbial Technology
%@ 0141-0229
%D 2005
