Evaluation of Allium hirtifolium effects on wound healing In rainbow trout ( oncorhynchus mykiss ) Ghodrati Azadi, H*1; Shahsavani, D1; Farhoodi, M2. 1School of Veterinary Medicine Ferdowsi University of Mashhad Iran. 2Vaccine and Serum Razi Research Institute, Mashhad, Iran. Objective: Cutaneous lesions in fish from the agents of viral, bacterial, parasitic, and mechanical and chemical agents is a very common infection that causes rapid and mortality is fish. Allium hirtifolium (Persian Shallot) belongs to Allium genus (Alliaceae family) and it is an Iranian traditional native herb which use as a condiment spice. It is well known in Iranian folk medicine and its bulbs have been widely used for treating rheumatic and inflammatory disorders. This study evaluated the influence of Allium hirtifolium on the healing process of experimentally wounded rainbow trout After usinf 5 mg/L every 48 h Allium hirtifolim as bath for 4 weeks, significant differences was observed in wound contraction in the test and control groups( p>0.05). Materials & Methods: In this study, 90 carp weighted average number of 60 to 70 grams, were randomly allocated into 1- the negative control (n=30) ,2- Allium hirtifolium treated (n=30) and 3- positve control as phenytoin treated (n=30) groups. They were distributed and spent a week to become compatible with the environment. To ensure the health of fish parasites and microbial assay in a number of them were done. To achieve the desired goals during the testing of a particular type of diet for each of the three groups were used. Fish were then experimentally wounded. Three duplicate treatment groups of fish were added experimental diets containing 5 mg /L Allium hirtifolium (NO:2) and 5 mg /L phenytoin(NO:3) every 48 h as bath water for 4 weeks, and sampled at 0, 3, 7, 10 and 21 days post-wounding for histological examination. Results & conclusion:The results confirmed a significant effect of shallot in the repair of cutaneous lesions in rainbow trout ( p>0.05). Allium hirtifolim can significantly stimulate epithelialization in full thickness wounds. Keywords: Allium hirtifolium, wound healing, rainbow trout ( oncorhynchus mykiss )

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