In a microbial fuel cell (MFC), microorganisms form an electrochemical system, in which sustainable production of green electricity is possible from a wide variety of substrates, ranging from acetate to complex organic wastes to cellulosic biomass. Our work was aimed to use dairy industry wastewater as the feed and investigate the effect of growing active biofilm in a double chamber MFC with a self-made fiber brush anode, where the stainless steel core of the brush was prependicular to the membrane surface. The voltage, current, resistance, and power density during the experiment were recorded. The results were expressed in form of polarization and power density curves in three time periods from the start of the experiment. The maximum power density of system at the first, second, and third periods were determined to be 16, 158, and 597 mW/m2, respectively. The significant progress in the performance of the MFC, highlighted the rule of biofilm formation in this system.

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