Objective: Amang the high incidence malignancies, urinary bladder carcinoma is the fourth and eighth most frequcnt among men and women, respectively. Transitional cell carcinomas (TCCs), which accuunts for 90% of bladder cancers, arise from the transitional epithelium of bladder. Bracken fern (Ptcridiul11 aquilinem) has been described as Onc of the most common plants on the planet. The consumption of bracken fern as food is associated with a high incidence of cancer in humans und animals. In cattle, brackcn induces can:inomas of urinary bladder causing bovine enzootic hcmaturia; this is a relativcly coml11on condition in brackeninfested pasturelands. Materials and Methods: The plant P. aquilinum was colft:cted from Gilan province and its dichloromethane extract was prepared. To study the cell cycle ofthe extract, TCC cells were treated with specific concentrations of the extract and examined for 24 hours aftcr treatment. The cell cycle was an~lysised using propidillm iodidestained nuclei. Results: Comparison survival percent TCC I:ells were treated with the extract, DMSO and unlTeated cells with used of eetl cycle assay show to an\'cst in a cell cycle of phase. Conclusion: Recent studies calTied out on the extracts from dilTerent P. aquilinulTI plants revealed at this plant has the potential of apoptosis and cell cycle arrest induction in cancer cells. The results of present· study confirmed the cytotoxic effects and cell cycle arrest of P. aquilinum on TCC cells

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