Today many researches are focused on the small fruits such as seedless barberry that are considered as source of organic and inorganic nutrients and medicinal compounds [1]. Qualtative indices of berries such as anthocyanins are affected by many invironmental factors as temperature and light [4,6]. Therefore, this expriment was conducted to evaluated the effects of variability of daily temperatures on qualitative indices of seedless barberry. The experiment was carried out as a split plot in time based on CRBD with three replications in Qayen, Southern Khorassan province, Iran, in year 2010. The experimental treatments were five different times of harvesting per day at 16 Sept. and 17 Oct., 2010. Harvesting was done every three hours which strated from 07.00 am (before sunrise) and ended at 19.00 pm (after sunset). Different hours temperatures were recorded at 16 Sept. and 17 Oct (Table 1). The qualitative indices such as pH, brix percenatge, acidity and anthocyanin were measured in each harvesting time. Results showed that qualitative indices of barberry fruits were improved in early morning and late afternoon, moreover, all of the indices were improved with delaying in harvesting date (Table 2). Many researches were shown that the amounts of anthocyanin, acidity and soluble solids are affected by changing temperature [3]. Anthocyanin and acidity had a decreasing trend with increasing of temperature (Table 1,2), that it seems to be related with decreasing of brix [5]. It has been reported that anthocyanins are more stable in low temperatures and high acidity [3,4] that observed before sunrise and after sunset.

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