The investigation was carried out to evaluate the effects of substrate and cultivar on biochemical characteristics of strawberry in soilless culture system. The experiment was conducted in a factorial experiment based on randomized complete design with four replications. Experimental treatments consisted of three strawberry cultivars (Camarosa, Mrak, and Selva) and six substrates (Rice hull, Sycamore pruning waste, Cocopeat + Perlite (50:50), Vermicomposts + Perlite + Cocopeat (5:45:50), (15:40:45), (25:35:40). Results of this experiment showed that the highest percentage of malformed fruits was observed in Selva cultivar in Rice hull (48.46%) and Camarosa cultivar in vermicompost + perlite + cocopeat (25:35:40) (48.46%), the highest of total anthocyanin content in Camarosa in vermicompost + perlite + cocopeat (15:40:45) (222.65 mg/100g), the highest of antioxidant in Camarosa and Mrak cultivars in substrates of Sycamore pruning waste and cocopeat + perlite (50:50) respectively (58.76% and 62% respectively), the highest of TSS in Selva in vermicompost + perlite + cocopeat (5:45:50) (8.66), the highest of Vitamin C in Selva in vermicompost + perlite + cocopeat (15:40:45) (108.05 mg/ 100cc), the highest of TA in Camarosa in vermicompost + perlite + cocopeat (5:45:50) (2.87 mg/100g). Response of each cultivar was different in each substrate. Therefore, the substrate affects on quality of fruit in strawberry and suitable choice of substrate is caused to production of desirable fruit.

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