Saffron (Crocus sativus L.) as an important growing in dry lands, have many clinical and additive effects so many studies are being done to light on clandestine aspects. Literatures indicate that peroxidase enzyme participate in many cellular processes such as auxin metabolism, wood formation, cell cement, response to external stresses and so on. In this study, the plant corms in two different months - JULY and NOVEMBER - and dormant and waking stages of saffron growth, sampled and then extracted based on partially purification with DEAE-Sephadex ion-exchange chromatography. Then, peroxidase extract analyzed quantitatively and qualitatively. To the end kinetic measurements were done according to different pH s and different substrate concentrations. As a notable result, distinctive differences were seen between peroxidase activity of dormant and waking saffron stages. Kinetic measurements revealed that there are different optimum pH and substrate concentrations for enzyme in two life periods of saffron corms. All the results are in coincide with other documents focused on cell metabolism and enzyme activities in growth periods of plants.

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