Title : ( Investigation of Equine herpesvirus-1 and 4 infections in equine population of Iran by real-time PCR )
Authors: Ali Sarani , Gholam Reza Mohammadi , Ashraf Mayameei , Masoud Akbari ,
Full TextAbstract
To detect the presence or absence of EHV-1 and EHV-4 in North-East equine population of Iran. Material and methods:Blood samples of 200 adult horses located in 80 different rural areas of North-East of Iran, were examined for Equine herpesvirus -1 and 4 presences.Absolute quantitation of EHV-4 target molecules was performed using standard curves and the detection limit of the assay was shown to be six copies per reaction. Results: Our study showed a high prevalence of EHV-4 (88%) in these regions. EHV-1 DNA was not detected in any sample. Conclusion: In addition to previous serological study, our report is the first to detect the EHVs in blood samples of Iran’s equine population by using a high sensitive real-time PCR diagnostic assay and it provides new information for the virus distribution map.
Keywords
, Equine herpesvirus, real-time PCR, virus quantitation.
@article{paperid:1033235,
author = {Sarani, Ali and Mohammadi, Gholam Reza and Mayameei, Ashraf and Masoud Akbari},
title = {Investigation of Equine herpesvirus-1 and 4 infections in equine population of Iran by real-time PCR},
journal = {Human & Veterinary Medicine},
year = {2013},
volume = {5},
number = {1},
month = {March},
issn = {2066-7655},
pages = {29--33},
numpages = {4},
keywords = {Equine herpesvirus; real-time PCR; virus quantitation.},
}
%0 Journal Article
%T Investigation of Equine herpesvirus-1 and 4 infections in equine population of Iran by real-time PCR
%A Sarani, Ali
%A Mohammadi, Gholam Reza
%A Mayameei, Ashraf
%A Masoud Akbari
%J Human & Veterinary Medicine
%@ 2066-7655
%D 2013
