Title : ( Improved salt tolerance in canola (Brasica napus) plants by overexpression of Arabidopsis Na/H antiporter gene AtNHX1 )
Authors: E. Doranie Uliaie , B. Ghareyazi , Mohammad Farsi , K. H. Kogel , J. Imani ,Access to full-text not allowed by authors
Abstract
A significant portion of the world cultivated land is affected by salinity reduces crop productivity in these areas. Breeding for salt tolerance is one of the important strategies to overcome this problem. Recently genetic engineering is becoming a promising approach to improving salt tolerance. In order to improve the yield performance of canola in saline soils, we transformed canola with Arabidopsis vacular Na? H antiporter gene AtNHX1 which enhances the plant capacity for reducing cytosolic Na by transporting Na into vacuole.Southern analysis of putative transgenic plants indicated that only one copy of the gene integrated into the plant genome. Overexpression of the AtNHX1 gene was shown in T1 transgenic plants. Under salinity conditions, stem and root lentgh and overall biomass of transgenic plants were significantkly higher compared to those of nontransgenic plants.
Keywords
, Genetic engineering, Na/H antiporter, Salat tolerance, Canola@article{paperid:1033695,
author = {E. Doranie Uliaie and B. Ghareyazi and Farsi, Mohammad and K. H. Kogel and J. Imani},
title = {Improved salt tolerance in canola (Brasica napus) plants by overexpression of Arabidopsis Na/H antiporter gene AtNHX1},
journal = {Journal of Plant Molecular Breeding},
year = {2012},
volume = {1},
number = {1},
month = {December},
issn = {2322-3332},
pages = {34--42},
numpages = {8},
keywords = {Genetic engineering; Na/H antiporter; Salat tolerance; Canola},
}
%0 Journal Article
%T Improved salt tolerance in canola (Brasica napus) plants by overexpression of Arabidopsis Na/H antiporter gene AtNHX1
%A E. Doranie Uliaie
%A B. Ghareyazi
%A Farsi, Mohammad
%A K. H. Kogel
%A J. Imani
%J Journal of Plant Molecular Breeding
%@ 2322-3332
%D 2012