Journal of Chromatography A, ( ISI ), Volume (107), No (1), Year (2005-1) , Pages (107-114)

Title : ( Automated analysis of individual particles using a commercial capillary electrophoresis system )

Authors: Hossein Ahmadzadeh ,

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Capillary electrophoretic analysis of individual submicrometer size particles has been previously done using custom-built instruments. Despite that these instruments provide an excellent signal-to-noise ratio for individual particle detection, they are not capable of performing automated analyses of particles. Here we report the use of a commercial Beckman P/ACE MDQ capillary electrophoresis (CE) instrument with on-column laser-induced fluorescence (LIF) detection for the automated analysis of individual particles. The CE instrument was modified with an external I/O board that allowed for faster data acquisition rates (e.g. 100 Hz) than those available with the standard instrument settings (e.g. 4 Hz). A series of eight hydrodynamic injections expected to contain 32±6 particles, each followed by an electrophoretic separation at −300Vcm−1 with data acquired at 100 Hz, showed 28±5 peaks corresponding to 31.9 particles as predicted by the statistical overlap theory. In contrast, a similar series of hydrodynamic injections followed by data acquisition at 4Hz revealed only 8±3 peaks suggesting that the modified system is needed for individual particle analysis. Comparison of electropherograms obtained at both data acquisition rates also indicate: (i) similar migration time ranges; (ii) lower variation in the fluorescence intensity of individual peaks for 100 Hz; and (iii) a better signal-to-noise ratio for 4Hz raw data. S/N improved for 100 Hz when data were smoothed with a binomial filter but did not reach the S/N values previously reported for post-column LIF detection. The proof-of-principle of automated analysis of individual particles using a commercially available CE system described here opens exciting possibilities for those interested in the study and analyses of organelles, liposomes, and nanoparticles.

Keywords

Individual particle analysis; Capillary electrophoresis; Fluorescence detection; Latex
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@article{paperid:1034062,
author = {Ahmadzadeh, Hossein},
title = {Automated analysis of individual particles using a commercial capillary electrophoresis system},
journal = {Journal of Chromatography A},
year = {2005},
volume = {107},
number = {1},
month = {January},
issn = {0021-9673},
pages = {107--114},
numpages = {7},
keywords = {Individual particle analysis; Capillary electrophoresis; Fluorescence detection; Latex microspheres},
}

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%0 Journal Article
%T Automated analysis of individual particles using a commercial capillary electrophoresis system
%A Ahmadzadeh, Hossein
%J Journal of Chromatography A
%@ 0021-9673
%D 2005

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