13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology , 2013-04-16

Title : ( Effects of hyaluronic acid on the behaviors of adipose-derived mesenchymal stem cells cultured on decellularized gingival matrix in vitro )

Authors: maryam alavi , Nasser Mahdavi SHahri , Maryam Moghaddam Matin , Masoud Fereidoni , Roya Lari , S. A. Banihashem Rad ,

Citation: BibTeX | EndNote

Background: Biological scaffolds derived from decellularized tissues and organs are used progressively in tissue engineering and regenerative medicine. Hyaluronic acid (HA) is a major component of extracellular matrix that regulates cellular behaviors during the morphogenesis, development and regenerative processes. Objectives: The effects of soaking decellularized gingival matrix in HA on the behaviors of adipose derived mesenchymal stem cells (AD-MSCs) aimed to be investigated by histological methods in the present research. Materials & Methods: After decellularization of human gingival tissue pieces using physical methods (slow and rapid freezing) and chemical agents sodium dodecyl sulfate (SDS) and Triton (X-100), washing and sterilization procedures were performed. Scaffolds were then divided into two groups including soaked scaffolds in HA (0.3% solution) and scaffolds without HA as controls. In the next step, scaffolds were seeded by a density of 6×105 AD-MSCs/cm2 in both groups. The scaffolds histological studies were performed after 1, 2, 3 and 4 weeks. Results: Histological studies revealed no changing in the cell density but increase in cell migration one and 2weeks of culture, while a decrease in cell density and migration was observed at 3rd and 4th weeks after culture in both groups but there were no significant differences in cell density and migration between the two groups. Conclusions: The evaluated concentration of HA in the present study did not show a significant impact on increasing the survival and migration of AD-MSCs.

Keywords

, Hyaluronic acid, Mesenchymal stem cells, Gingival, tissue, Decellularization, Tissue
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@inproceedings{paperid:1034235,
author = {Alavi, Maryam and Mahdavi SHahri, Nasser and Moghaddam Matin, Maryam and Fereidoni, Masoud and Lari, Roya and S. A. Banihashem Rad},
title = {Effects of hyaluronic acid on the behaviors of adipose-derived mesenchymal stem cells cultured on decellularized gingival matrix in vitro},
booktitle = {13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology},
year = {2013},
location = {یزد, IRAN},
keywords = {Hyaluronic acid; Mesenchymal stem cells; Gingival; tissue; Decellularization; Tissue engineering},
}

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%0 Conference Proceedings
%T Effects of hyaluronic acid on the behaviors of adipose-derived mesenchymal stem cells cultured on decellularized gingival matrix in vitro
%A Alavi, Maryam
%A Mahdavi SHahri, Nasser
%A Moghaddam Matin, Maryam
%A Fereidoni, Masoud
%A Lari, Roya
%A S. A. Banihashem Rad
%J 13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology
%D 2013

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