Title : ( Preparation and evaluation of a three-dimensional natural bioscaffold from human gingival stroma for tissue engineering )
Authors: maryam alavi , Nasser Mahdavi SHahri , Maryam Moghaddam Matin , Masoud Fereidoni , Roya Lari , S. A. Banihashem Rad ,
Full TextAbstract
Background: Bio scaffold technology is expected to revolutionize the therapeutic procedures in the next decades by providing a rapid solution to tissue replacement needs. Collagen bio scaffolds give promising results in oral mucosa engineering due to high biocompatibility and biodegradability of collagen. Objectives: The objective of this study was to prepare a natural scaffold mainly containing collagen from human gingival stroma and evaluate maintenance and migration of human adipose derived mesenchymal stem cells (AD-MSCs) cultured on the scaffold. Materials & Methods: Cellular components of human gingival stroma were removed with snap freez thaw and administration of two detergents including sodium dodecyl sulfate (SDS) and Triton X-100. Collagen contents of the bio scaffolds were studied by Van Gieson’s staining. Bio scaffolds were then seeded by AD-MSCs, and investigated histologically for 4 weeks. Results: The histological studies showed that collagen fibers in connective tissue remained intact in the scaffolds. In microscopic studies, adhesion, penetration and migration of human AD-MSCs were observed on the scaffolds. Conclusions: According to the results, it can be concluded that gingival bio scaffold may have inductive effects on cellular behaviors such as adhesion and migration of human AD-MSCs and is a suitable model for future studies in tissue engineering.
Keywords
, Human gingiva, Collagen, Bioscaffold, Mesenchymal stem cells, Tissue engineering
@inproceedings{paperid:1034236,
author = {Alavi, Maryam and Mahdavi SHahri, Nasser and Moghaddam Matin, Maryam and Fereidoni, Masoud and Lari, Roya and S. A. Banihashem Rad},
title = {Preparation and evaluation of a three-dimensional natural bioscaffold from human gingival stroma for tissue engineering},
booktitle = {13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology},
year = {2013},
location = {یزد, IRAN},
keywords = {Human gingiva; Collagen; Bioscaffold; Mesenchymal stem cells; Tissue engineering},
}
%0 Conference Proceedings
%T Preparation and evaluation of a three-dimensional natural bioscaffold from human gingival stroma for tissue engineering
%A Alavi, Maryam
%A Mahdavi SHahri, Nasser
%A Moghaddam Matin, Maryam
%A Fereidoni, Masoud
%A Lari, Roya
%A S. A. Banihashem Rad
%J 13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology
%D 2013
