Background: Bio scaffold technology is expected to revolutionize the therapeutic procedures in the next decades by providing a rapid solution to tissue replacement needs. Collagen bio scaffolds give promising results in oral mucosa engineering due to high biocompatibility and biodegradability of collagen. Objectives: The objective of this study was to prepare a natural scaffold mainly containing collagen from human gingival stroma and evaluate maintenance and migration of human adipose derived mesenchymal stem cells (AD-MSCs) cultured on the scaffold. Materials & Methods: Cellular components of human gingival stroma were removed with snap freez thaw and administration of two detergents including sodium dodecyl sulfate (SDS) and Triton X-100. Collagen contents of the bio scaffolds were studied by Van Gieson’s staining. Bio scaffolds were then seeded by AD-MSCs, and investigated histologically for 4 weeks. Results: The histological studies showed that collagen fibers in connective tissue remained intact in the scaffolds. In microscopic studies, adhesion, penetration and migration of human AD-MSCs were observed on the scaffolds. Conclusions: According to the results, it can be concluded that gingival bio scaffold may have inductive effects on cellular behaviors such as adhesion and migration of human AD-MSCs and is a suitable model for future studies in tissue engineering.

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