Title : ( A multiple-capillary electrophoresis system for smallscale DNA sequencing and analysis )
Authors: Hossein Ahmadzadeh ,
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Abstract
A five-capillary system has been developed for DNA sequencing and analysis. The post-column fluorescence detector is based on a sheath-flow cuvette. The instrument provides uniform and continuous illumination of the samples. The cuvette virtually eliminates cross-talk in the fluorescence signal between capillaries. Discrete single-photon counting avalanche photodiodes provide high efficiency light detection. The instrument has detection limits (3σ) of 130 ± 30 fluorescein molecules injected onto each capillary. Over 650 bases of sequence at 98.8% accuracy were generated in 100 min at 50°C from M13mp18. Separation and detection of short tandem repeats proved efficient and accurate with the use of internal standards for direct comparison of migration times between capillaries.
Keywords
, Multiple, capillary electrophoresis; DNA sequencing and analysis
@article{paperid:1034670,
author = {Ahmadzadeh, Hossein},
title = {A multiple-capillary electrophoresis system for smallscale DNA sequencing and analysis},
journal = {Nucleic Acids Research},
year = {1999},
volume = {27},
number = {24},
month = {December},
issn = {0305-1048},
pages = {1--7},
numpages = {6},
keywords = {Multiple-capillary electrophoresis; DNA sequencing and analysis},
}
%0 Journal Article
%T A multiple-capillary electrophoresis system for smallscale DNA sequencing and analysis
%A Ahmadzadeh, Hossein
%J Nucleic Acids Research
%@ 0305-1048
%D 1999
