Journal Chromatography B, ( ISI ), Volume (780), No (1), Year (2002-3) , Pages (283-287)

Title : ( H eterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde )

Authors: Alexander V. Stoyanov , Hossein Ahmadzadeh , Sergey N. Krylov ,

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Fluorogenic reagents are used for protein labeling when high-sensitivity fluorescence detection is required. Similar to traditional labeling with activated fluorescent dyes, such as fluorescein isothiocyanate, a fluorogenic reaction is expected to change the physical–chemical properties of proteins. Knowledge of these changes may be essential for efficient separation and identification of labeled proteins. Here we studied the effect of labeling of myoglobin with a fluorogenic reagent on the acid–base properties of the protein. The fluorogenic reagent used was 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ). In slab-gel isoelectric focusing, we found that the labeling reaction generated at least six species with pI values lower than that of non-labeled myoglobin. These species can be identified as products of progressive labeling of myoglobin with one to six FQ molecules. The same series of FQ-labeled species were observed when the reaction products were analyzed by capillary zone electrophoresis. The comparison of experimental and theoretical pI values allowed us to elucidate the labeling pattern—the number of FQ molecules corresponding to each labeled product detected by isoelectric focusing.

Keywords

Proteins;
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@article{paperid:1034671,
author = {Alexander V. Stoyanov and Ahmadzadeh, Hossein and Sergey N. Krylov},
title = {H eterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde},
journal = {Journal Chromatography B},
year = {2002},
volume = {780},
number = {1},
month = {March},
issn = {1570-0232},
pages = {283--287},
numpages = {4},
keywords = {Proteins; 3-(2-furoyl)quinoline-2-carboxaldehyde},
}

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%0 Journal Article
%T H eterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde
%A Alexander V. Stoyanov
%A Ahmadzadeh, Hossein
%A Sergey N. Krylov
%J Journal Chromatography B
%@ 1570-0232
%D 2002

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