Analytical and Bioanalytical Chemistry, ( ISI ), Volume (384), No (3), Year (2006-1) , Pages (169-174)

Title : ( On-column labeling for capillary electrophoretic analysis of individual mitochondria directly sampled from tissue cross sections )

Authors: Hossein Ahmadzadeh , LaDora V. Thompson , Edgar A. Arriaga ,

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This technical note reports on a new procedure to on-column-label organelles sampled from a tissue cross section into a fused silica capillary. These organelles are then analyzed by capillary electrophoresis with postcolumn laser-induced fluorescence detection. In this procedure, the fluorescent label does not come in contact with the tissue, which facilitates visualization of the sampled tissue cross section. In addition, on-column labeling allows for better control of the reaction time and fluorescent label concentrations. As a proof-of-principle, we show results of mitochondria from rat gastrocnemius muscle cross sections that were on-column-labeled with 10-N-nonyl acridine orange (NAO), a mitochondrion-specific probe, and compare them with results for NAO in-tissue labeling of the same tissue. The new organelle labeling procedure reported here may easily be extended to the analysis of individual organelles in other biological samples and may become a valuable tool in studies investigating the role of mitochondria in muscle aging and exercise physiology.

Keywords

, On, column labeling ;Capillary electrophoresis ; Fluorescence ; Mitochondria ;
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@article{paperid:1034678,
author = {Ahmadzadeh, Hossein and LaDora V. Thompson and Edgar A. Arriaga},
title = {On-column labeling for capillary electrophoretic analysis of individual mitochondria directly sampled from tissue cross sections},
journal = {Analytical and Bioanalytical Chemistry},
year = {2006},
volume = {384},
number = {3},
month = {January},
issn = {1618-2642},
pages = {169--174},
numpages = {5},
keywords = {On-column labeling ;Capillary electrophoresis ; Fluorescence ; Mitochondria ; Muscle},
}

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%0 Journal Article
%T On-column labeling for capillary electrophoretic analysis of individual mitochondria directly sampled from tissue cross sections
%A Ahmadzadeh, Hossein
%A LaDora V. Thompson
%A Edgar A. Arriaga
%J Analytical and Bioanalytical Chemistry
%@ 1618-2642
%D 2006

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