In vitro and in vivo culture of Z. jujuba seeds under stratification and scarification were carried out. Sulfuric acid 98% (H2SO4) exposure time (0, 5, 10 and 15 minutes), stratification and scarification (acid scarification; 0, 5, 10 and 15 minutes and mechanical scarification) were used for in vitro and in vivo conditions, respectively. In in vitro culture, Germination percentage, vigor index and the rootlet number increased by ascending acid exposure time while the highest of seedling length was obtained in 5 minutes acid exposure. In in vivo culture, stratification let to decrease of seedling and radicle length and mechanical scarification raised germination percentage, vigor index, seedling length, radicle length, rootlet number and rootlet length. Stratification plus mechanical scarification had the highest the rootlet number and length whereas other characteristics had the highest amount on mechanical scarification without stratification in the stratification × scarification interactions. No germination occurred in 15 minute acid exposure in vivo culture.

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