Vitex pseudo-negundo (VPN) and Calotropis persica (CP) are the native plants in the northeast and south of Iran. In the present study, after collecting and drying the seeds, essential oil extracted by steam distillation and then anti-microbial effects of these essential oil were investigated according to serial dilution on six microorganisms that are: E.coli, Pseudomonas aerogenosa, Entrobacter sp., Entrococcus, Staph saprophyticus and Candida albicans in test tube and then in solid medium of blood agar for 6 months with repetitions [1]. The conclusions of each six repetitions showed that minimal dilution of the essential oil which was able to control the bacterial growth was 1: 32 (on Psedomonas and E. coli for VPN and CP, respectively) and maximal dilution is 1: 128 (on E. coli and Candida albicans for VPN) and 1: 256 (on Entrobacter for CP).

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