In this study the effects of nitrogen bio fertilizer (inoculation with a mixture of Azotobacter and Azospirillum), bio sulfur (inoculation with sulfur solubilizing bacterium Thiobacillus), super absorbent polymer and their intraction on content and constituents of essential oil of Ocimum ciliatum was investigated. The experiment was carried out at randomized complete block design with four replications and eight treatments. The results indicated that bio fertilizers and super absorbent polymer did not affect the essential oil content of basil as compared to the control but oil composition using GC and GC-MS revealed the volatile oil composition was affected as a result of applying various treatments also concentration of component influenced by fertilization of basil plants with bio fertilizers and super absorbent polymer. The main components of Ocimum ciliatum were methyl chavicol and citral (neral + geranial) that the contents of them had an opposite trend with each other. Application of bio fertilizers gave the highest content of methyl chavicol (67.1%) while the maximum values of neral (16.2%) in bio sulfur and super asorbent mixture and geranial (22.2%) in bio sulfur compared to the other treatments reported and the lowest content of both constituents was obtained in bio fertilizers treatments. In this study, the dominant compound presented under all treatments was aromatic compound, its content did not have a constant trend but the highest content of this group (68.8%) obtained in mixture of bio fertilizers. Monoterpene and sesquiterpene compounds were decreased and increased as a result of using bio fertilizers and super absorbent treatments, respectively.

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