13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology , 2013-04-16

Title : ( Strategies for simulation of tendon by human Adipose-Derived Mesenchymal Stem Cells seeded on engineered tendon matrix )

Authors: mahboubeh hezbollahi , Nasser Mahdavi SHahri , Maryam Moghaddam Matin , Roya Lari ,

Citation: BibTeX | EndNote

The recent advances in stem cell biology and tissue engineering are becoming progressivly oriented toward biologically cellular microenvironments to improve stem cell growth, differentiation, and functional assembly. The ideal microenvironment for tendon engineering would possess the basic structure of the tendon, native extracellular matrix (ECM), and capability of cell seeding. The purpose of this study was to evaluate the potential of the engineered tendon matrix (ETM) as a suitable scaffold for seeding human adipose- derived mesenchymal stem cells on it. tendon was decellularized by physical, enzymatic and chemical methods by using liquid nitrogen, trypsin and Triton X-100 respectively. In order to preparation biofilm, decellularized tendon matrix was treated with acid acetic and added to a Petri dish. The labeled human Adipose-Derived Mesenchymal Stem Cells (hASCs) with GFP (green fluorescent protein) were seeded on it. The result of this study showed that hASCs could attach and preserved on the biofilm. Therefore the biofilm derived ETM has the ability to preserve the native extracellular matrix, and also has the capability for cell seeding

Keywords

, Cellular microenvironment, Tendon matrix, adipose derived mesenchymal stem
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@inproceedings{paperid:1035479,
author = {Hezbollahi, Mahboubeh and Mahdavi SHahri, Nasser and Moghaddam Matin, Maryam and Lari, Roya},
title = {Strategies for simulation of tendon by human Adipose-Derived Mesenchymal Stem Cells seeded on engineered tendon matrix},
booktitle = {13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology},
year = {2013},
location = {یزد, IRAN},
keywords = {Cellular microenvironment; Tendon matrix; adipose derived mesenchymal stem cell},
}

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%0 Conference Proceedings
%T Strategies for simulation of tendon by human Adipose-Derived Mesenchymal Stem Cells seeded on engineered tendon matrix
%A Hezbollahi, Mahboubeh
%A Mahdavi SHahri, Nasser
%A Moghaddam Matin, Maryam
%A Lari, Roya
%J 13th Iranian Congress of Biochemistry and 5th International Congress of Biochemistry and Molecular Biology
%D 2013

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