The recent advances in stem cell biology and tissue engineering are becoming progressivly oriented toward biologically cellular microenvironments to improve stem cell growth, differentiation, and functional assembly. The ideal microenvironment for tendon engineering would possess the basic structure of the tendon, native extracellular matrix (ECM), and capability of cell seeding. The purpose of this study was to evaluate the potential of the engineered tendon matrix (ETM) as a suitable scaffold for seeding human adipose- derived mesenchymal stem cells on it. tendon was decellularized by physical, enzymatic and chemical methods by using liquid nitrogen, trypsin and Triton X-100 respectively. In order to preparation biofilm, decellularized tendon matrix was treated with acid acetic and added to a Petri dish. The labeled human Adipose-Derived Mesenchymal Stem Cells (hASCs) with GFP (green fluorescent protein) were seeded on it. The result of this study showed that hASCs could attach and preserved on the biofilm. Therefore the biofilm derived ETM has the ability to preserve the native extracellular matrix, and also has the capability for cell seeding

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