Finding antimicrobial compound with low side effects is important because of microbial antibiotics resistance. The gram positive bacterium such as Staphylococcus aureus is mainly responsible for post operative wound infections, toxic shock syndrome, endocarditis,osteomyelitis and food poisoning[1]. In this study, mangrove leaves were dried in shadow and appropriate condition. After extraction with water, antimicrobial effect of extract were determined by \\\\\\\"screening antibacterial activity\\\\\\\" and \\\\\\\"disk agar diffusion test\\\\\\\" in 20, 40, 60 and 80 Percent concentration of the extract against Staphylococcus aureus PTCC 2592 . The results showed that mangrove leaf extract in screening antibacterial activity method in 2000 μg/ml, inhibit Staphylococcus aureus growth. In \\\\\\\"disk agar diffusion test, mangrove watery extract, in 60 and 80 percent concentration ,the mentioned extract have shown inhibition of pathogen growth, but there was no inhibitry on 20 and 40 percent. Results showed mangrove extract can be used as natural antimicrobial in food products.

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