The ancient-Egyptians were familiar with many medicinal herbs and were aware of their usefulness in treatment of various diseases [1]. Some serotypes of E. coli can cause serious food poisoning in humans, and are occasionally responsible for product recalls due to food contamination. In this study, mangrove leaves were dried in Shadow and appropriate condition. After extraction with ethanol 96 degree, antimicrobial effect were determined by \"screening antibacterial activity\" and \"disk agar diffusion test \" at 20, 40, 60 and 80 Percent concentration of the extract against E coli PTCC 1330. The results show that alcoholic extract in screening antibacterial activity method at 2000 μg/ml, couldn\'t inhibit gram negative bacteria growth. In disk agar diffusion test, alcoholic extract have shown inhibition in 60 and 80 percent concentration of the extract but there was no inhibitory effect at 20 and 40 percent. Results showed mangrove extract can be used as natural antimicrobial in food products.

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